Authors: Charles L. Wilkins, James D. Winefordner, Jackson O. Lay
ISBN-13: 9780471654421, ISBN-10: 0471654426
Format: Hardcover
Publisher: Wiley, John & Sons, Incorporated
Date Published: December 2005
Edition: 1st Edition
CHARLES L. WILKINS, PhD, is a Distinguished Professor in the Department of Chemistry and Biochemistry at the University of Arkansas. He is the author or coauthor of over 250 scientific publications and has received numerous awards for his research.
JACKSON O. LAY, Jr., PhD, is the Director of the Arkansas Statewide Mass Spectrometry Facility at the University of Arkansas, and the author or coauthor of more than 100 publications in the field. In addition to his work with bacteria, he has developed mass spectrometry methods for cancer research and toxicology.
A multidisciplinary approach to understanding the fundamentals of mass spectrometry for bacterial analysis
From chemotaxonomy to characterization of targeted proteins, Identification of Microorganisms by Mass Spectrometry provides an overview of both well-established and cutting-edge mass spectrometry techniques for identifying microorganisms. A vital tool for microbiologists, health professionals, and analytical chemists, the text is designed to help scientists select the most effective techniques for use in biomedical, biochemical, pharmaceutical, and bioterror defense applications.
Since microbiological applications of mass spectrometry require a basic understanding of both microbiology and analytical chemistry, the editors have incorporated material from both disciplines so that readers from either field will come to understand the necessary principles of the other. Featuring contributions from some of the most recognized experts in both fields, this volume provides specific examples of fundamental methods as well as approaches developed in the last decade, including:
Identification of Microorganisms by Mass Spectrometry represents the most comprehensive and up-to-date work on the topic currently available. It is liberally illustrated with figures and tables and covers every aspect of spectrometric identification of microorganisms, including experimental procedures, various means of sample preparation, data analysis, and interpretation of complex mass spectral data.
1 | Cultural, serological, and genetic methods for identification of bacteria | 1 |
2 | Mass spectrometry : identification and biodetection, lessons learned and future developments | 23 |
3 | An introduction to MALDI-TOF MS | 39 |
4 | The development of the block II chemical biological mass spectrometer | 61 |
5 | Method reproducibility and spectral library assembly for rapid bacterial characterization by metastable atom bombardment pyrolysis mass spectrometry | 91 |
6 | MALDI-TOF mass spectrometry of intact bacteria | 125 |
7 | Development of spectral pattern-matching approaches to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for bacterial identification | 153 |
8 | Studies of malaria by mass spectrometry | 161 |
9 | Bacterial strain differentiation by mass spectrometry | 181 |
10 | Bacterial protein biomarker discovery : a focused approach to developing molecular-based identification systems | 203 |
11 | High-throughput microbial characterizations using electrospray ionization mass spectrometry and its role in functional genomics | 229 |
12 | Bioinformatics for flexibility, reliability, and mixture analysis of intact microorganisms | 257 |
13 | MALDI-FTMS of whole-cell bacteria | 279 |
14 | A review of antibody capture and bacteriophage amplification in connection with the direct analysis of whole-cell bacteria by MALDI-TOF-MS | 301 |
15 | Discrimination and identification of microorganisms by pyrolysis mass spectrometry : from burning ambitions to cooling embers - a historical perspective | 319 |