Authors: Connie R. Mahon, Donald C. Lehman, George Manuselis Jr.
ISBN-13: 9781416061656, ISBN-10: 1416061657
Format: Hardcover
Publisher: Elsevier Health Sciences
Date Published: March 2010
Edition: 4th Edition
Mahon, Connie R., MS, MT (ASCP), CLS (Univ of Texas Health Science Center at San Antonio); Manuselis, George, MA, MT (ASCP) (Univ of Akron)
Providing a solid introduction to the essentials of diagnostic microbiology, this accessible, full-color text helps you develop the problem-solving skills necessary for success in the clinical setting. A reader-friendly, "building block" approach to microbiology moves progressively from basic concepts to advanced understanding, guiding you through the systematic identification of etiologic agents of infectious diseases.
This is a new textbook addressing the basics in the practice of clinical microbiology and the identification of microorganisms causing infectious diseases. This text was designed for the entry level laboratory scientist to provide a firm theoretical foundation in microbiology and the skills required in the clinical laboratory. These objectives are worthwhile because the educational background of students varies widely. The target audience is the medical technologist learning practical skills to work in a diagnostic laboratory. It is also appropriate for pathology residents and infectious disease fellows who are doing a rotation in clinical microbiology. There are a few minor errors in the material resulting from generalizations and the lack of space to discuss certain concepts in detail. Most contributors are well known in their fields of expertise and cover the material very thoroughly. The book is written in a user-friendly manner with colorful topic headers and chart titles. Topics are divided into short sections for quick reference and easy reading. There are no long paragraphs of text to wade thorough to find the area of interest. The most outstanding feature of the book is the extensive illustrations and high quality photographs to demonstrate the appearance of an organism, a test result, or an instrument under discussion. Because the text is more than 1,000 pages, the well-arranged table of contents is a critical feature. The majority of the references are review articles written in the last 10 years. This textbook would be a welcome addition to any clinical microbiology lab for the continued training of new personnel and the reeducation of current staff. It covers multiple facetsof the microbiology lab from biosafety practices and quality improvement to new technologies such as PCR. The approach is practical with sample charts and tables ready to use in one's own laboratory.
Pt. I | Introduction to Clinical Microbiology | 1 |
Ch. 1 | Bacterial Cell Structure, Physiology, Metabolism, and Genetics | 3 |
Ch. 2 | Control of Microorganisms | 25 |
A | Disinfection and Sterilization | 25 |
B | Microbiology Laboratory Safety | 32 |
Ch. 3 | Concepts in Antimicrobial Therapy | 49 |
A | Antimicrobial Mechanisms of Action | 49 |
B | Procedures in Antimicrobial Susceptibility Testing | 58 |
C | Special Antimicrobial Susceptibility Tests | 89 |
Ch. 4 | Quality Improvement in the Microbiology Laboratory | 97 |
A | Quality Issues in Clinical Microbiology | 97 |
B | Putting the Laboratory Test to the Test | 111 |
Ch. 5 | Emergent Technologies | 117 |
Introduction | 118 | |
A | Direct Microbial Antigen Detection | 119 |
B | Serologic Diagnosis of Infectious Diseases | 141 |
C | Rapid Methods and Automation in the Microbiology Laboratory | 161 |
D | Identification of Microorganisms Using Chromatographic Techniques | 168 |
E | Diagnostic Applications of DNA Probes | 185 |
F | Diagnostic Applications of Polymerase Chain Reaction | 197 |
Ch. 6 | Host-Parasite Interaction | 213 |
A | Normal Microbial Flora | 213 |
B | Pathogenesis of Infection | 217 |
Ch. 7 | General Concepts in Specimen Collection and Handling | 235 |
Ch. 8 | Microscopic Examination of Infected Materials | 257 |
Ch. 9 | Utilization of Colonial Morphology for the Presumptive Identification of Microorganisms | 307 |
Ch. 15 | Haemophilus and Other Fastidious Gram-Negative Rods | 417 |
A | Haemophilus, Pasteurella, Brucella, and Francisella | 417 |
B | Legionella | 430 |
C | Bordetella | 440 |
Ch. 16 | Enterobacteriaceae | 447 |
Ch. 17 | Vibrio, Aeromonas, Plesiomonas, and Campylobacter | 491 |
Ch. 18 | Nonfermenting Gram-Negative Bacilli and Miscellaneous Gram-Negative Rods | 513 |
Ch. 19 | Anaerobes of Clinical Importance | 539 |
Ch. 20 | The Spirochetes | 595 |
Ch. 21 | Chlamydia, Mycoplasma, and Ureaplasma | 607 |
A | Chlamydia | 607 |
B | Mycoplasma and Ureaplasma | 622 |
Ch. 22 | Mycobacterium Tuberculosis and Other Nontuberculous Mycobacteria | 635 |
Ch. 23 | Medically Significant Fungi | 677 |
Ch. 24 | Diagnostic Parasitology | 725 |
Ch. 25 | Clinical Virology | 795 |
Pt. III | Laboratory Diagnosis of Infectious Diseases: An Organ System Approach to Diagnostic Microbiology | 827 |
Ch. 26 | Upper and Lower Respiratory Tract Infections | 829 |
Ch. 27 | Skin and Soft-Tissue Infections | 869 |
Ch. 28 | Gastrointestinal Infections and Food Poisoning | 893 |
Ch. 29 | Infections of the Central Nervous System | 915 |
Ch. 30 | Bacteremia | 935 |
Ch. 31 | Urinary Tract Infections | 949 |
Ch. 32 | Sexually Transmitted Diseases | 971 |
Ch. 33 | Infections in Special Patient Populations | 983 |
Ch. 34 | Zoonotic and Rickettsial Infections | 991 |
Ch. 35 | Ocular Infections | 1019 |
Appendix A: Selected Bacteriologic Culture Media, Stains, and Reagents | 1049 | |
Appendix B: Selected Mycologic Media and Stains | 1073 | |
Appendix C: Nomenclature Changes for the Enterobacteriaceae and Nonfermentative Bacilli | 1076 | |
Index | 1077 |