Authors: Donald M. Crothers, Victor A. Bloomfield, Ignacio Tinoco
ISBN-13: 9780935702491, ISBN-10: 0935702490
Format: Hardcover
Publisher: University Science Books
Date Published: March 2000
Edition: (Non-applicable)
Nucleic Acids presents an up-to-date and comprehensive account of the structures and physical chemistry properties of nucleic acids, with special emphasis on biological function. With a targeted audience of:
1)molecular biologists
2)physical biochemists
3)physical chemists
The book has been carefully organized to reach three different audiences while requiring only introductory physical chemistry and molecular biology as prerequisites.
Nucleic Acids will serve as a textbook in physical biochemistry and biophysical chemistry classes, as well as a supplemental text in courses on nucleic acid biochemistry or molecular biology, and as a personal reference for students and researchers in these fields.
Key Features:
Provides a detailed account of the structures and physical chemistry properties of nucleic acids, with an emphasis on the implications for biological function. The volume is organized to be of use to physical chemists, physical biochemists, and molecular biologists as well as students in those disciplines, requiring only a basic understanding of physical chemistry and molecular biology as prerequisites. Each chapter begins with a statement of the biological significance of each topic, followed by a presentation of the basic physical ideas and major results. Quantitative details are developed in special sections or appendices. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Preface | ix | |
Chapter 1 | Introduction | 1 |
1. | Biological Roles of the Nucleic Acids | 1 |
2. | Physical Chemistry and Nucleic Acid Function | 2 |
3. | Outline of this Book | 3 |
4. | Obtaining Nucleic Acids for Physical Chemical Study | 6 |
5. | The Periodical Literature of Nucleic Acids | 7 |
6. | Books and Monographs | 8 |
7. | Computer Analysis of Sequences and Structures | 10 |
References | 10 | |
Chapter 2 | Bases, Nucleosides, and Nucleotides | 13 |
1. | Structures | 13 |
2. | Electron Distributions | 22 |
3. | Base Pairing and Base Stacking | 31 |
References | 41 | |
Chapter 3 | Chemical and Enzymatic Methods | 45 |
1. | Introduction | 45 |
2. | Synthesis and Hydrolysis | 46 |
3. | Determination of Sequence | 52 |
4. | Determination of Secondary Structure | 58 |
5. | Determination of Tertiary Structure | 69 |
References | 72 | |
Chapter 4 | Nucleic Acid Structures from Diffraction Methods | 79 |
1. | Introduction | 79 |
2. | Diffraction Methods | 80 |
3. | General Features of Duplex DNA and RNA Structure | 87 |
4. | Detailed Structure and Sequence Dependence of Double-Stranded DNA | 93 |
5. | Double-Helical RNA Duplex | 100 |
6. | DNA Helices with Imperfect Base Pairing | 101 |
7. | Local Mobility of Double Helical Nucleic Acids | 102 |
8. | Transfer RNA | 103 |
References | 108 | |
Chapter 5 | Structure and Dynamics by NMR | 111 |
1. | Introduction to NMR Methods | 112 |
2. | Systematic Assignment of Resonances | 122 |
3. | Parameters for Structure Analysis | 134 |
4. | Characterization of Structures | 141 |
5. | Dynamic Analysis | 146 |
6. | Examples of NMR Studies of Nucleic Acids | 150 |
7. | Conclusions | 158 |
References | 158 | |
Chapter 6 | Electronic and Vibrational Spectroscopy | 165 |
1. | Electronic Absorption Spectra in the Ultraviolet | 166 |
2. | Fluorescence and Phosphorescence | 181 |
3. | Circular Dichroism | 185 |
4 | Linear Dichroism | 199 |
5. | Infrared Absorption and Raman Scattering | 207 |
References | 217 | |
Chapter 7 | Theoretical Methods | 223 |
1. | Theoretical Foundations | 223 |
2. | Simulation Methods | 230 |
3. | Theoretical Rationale for Conformational Preferences of Nucleic Acid Components | 239 |
4. | Sequence-Dependent Effects within Helices | 242 |
5. | Mismatches, Damaged DNA, and DNA-Drug Interactions | 249 |
6. | Concluding Perspectives | 253 |
References | 254 | |
Chapter 8 | Conformational Changes | 259 |
1. | Single-Strand Stacking | 261 |
2. | Double Helix Formation by Oligonucleotides without Loops | 271 |
3. | Double Helix Formation by Oligonucleotides with Loops | 291 |
4. | Double Helix Formation with Polynucleotides | 302 |
5. | Environmental Effects on Helix Stability | 307 |
6. | Triple Helixes | 310 |
7. | G-Quartets | 311 |
8. | Predicting Secondary Structure | 312 |
9. | Predicting Three-Dimensional Structure | 315 |
10. | Helix-Helix Transitions | 317 |
Appendix | 317 | |
References | 321 | |
Chapter 9 | Size and Shape of Nucleic Acids in Solution | 335 |
1. | Characterization at the Macromolecular Level | 336 |
2. | Models for Molecular Structure | 337 |
3. | Frictional Coefficients for Model Shapes | 341 |
4. | Electrophoresis | 345 |
5. | Sedimentation Velocity | 354 |
6. | Diffusion | 360 |
7. | Sedimentation Equilibrium | 364 |
8. | Viscosity | 368 |
9. | Viscoelasticity | 369 |
10. | Rotational Dynamics | 372 |
11. | Scattering | 381 |
12. | Dynamic Light Scattering | 390 |
13. | Microscopy: Visualization of Single Molecules | 396 |
14. | Bending and Twisting of DNA | 407 |
References | 433 | |
Chapter 10 | Supercoiled DNA | 443 |
1. | Introduction | 443 |
2. | Topoisomerases and Gyrases | 444 |
3. | Geometry and Topology of DNA Supercoiling | 446 |
4. | Energetics of Twisting and Bending | 463 |
5. | Stress-Induced Reactions | 466 |
References | 470 | |
Chapter 11 | Interaction of Nucleic Acids and Water and Ions | 475 |
1. | Hydration | 476 |
2. | Polyelectrolyte Behavior | 488 |
3. | Hydration in Ionic Solutions | 504 |
4. | Specific Binding of Metal Ions and Polyamines | 507 |
5. | Mixed Aqueous-Nonaqueous Solvents | 521 |
Appendix | 522 | |
References | 528 | |
Chapter 12 | Interaction and Reaction with Drugs | 535 |
1. | Modes of Interaction: Intercalation and Groove Binding | 536 |
2. | Theoretical Description of Binding Equilibria | 537 |
3. | Experimental Studies of Binding Equilibria | 547 |
4. | Solution Studies of the Structure of Drug-DNA Complexes | 565 |
5. | Kinetics of Ligand Binding | 571 |
6. | Natural Products that React Covalently with DNA | 575 |
7. | Synthetic DNA Binding Agents | 585 |
References | 591 | |
Chapter 13 | Protein-Nucleic Acid Interactions | 597 |
1. | Biological Role of Protein-Nucleic Acid Interactions | 598 |
2. | Elementary Energetics and Specificity of Interaction | 601 |
3. | DNA Sequence Motifs | 615 |
4. | Twisting and Bending of DNA Sequences in Protein Complexes | 626 |
5. | Looping of DNA Sequences | 641 |
6. | Structural Motifs in Sequence Specific DNA-Protein Interactions | 645 |
7. | Motifs in RNA-Protein Interactions | 677 |
8. | Structural Motifs in Enzymes that Act on Nucleic Acids | 683 |
9. | Solution Characterization of Binding Interactions | 692 |
10. | Kinetics and the Search Mechanism | 704 |
References | 711 | |
Chapter 14 | Higher Order Structure | 725 |
1. | DNA Condensation in Vitro | 725 |
2. | DNA Packaging in Bacteriophage and Bacteria | 749 |
3. | DNA Packaging in Eukaryotes | 756 |
References | 766 | |
Index | 775 |