Laboratory Experiments in Microbiology » (9th Edition)

Ted R. Johnson is a professor of biology at St. Olaf College, a liberal arts college in Northfield, Minnesota, where he teaches courses in microbiology and immunology. He has taught at St. Olaf for 31 years and previously taught at Mankato State University. He received his master’s degree and his Ph.D in microbiology from the University of Illinois in Chicago, Illinois. While at St. Olaf College, he has developed and directed several abroad semester and interim programs. His research focuses on the immune response to virally induced cancer in various animal models.

Christine L. Case is a registered microbiologist and a professor of microbiology at Skyline College in San Bruno, California, where she has taught for the past 38 years. She received her Ed. D. in curriculum and instruction from Nova Southeastern University and her M.A. in microbiology from San Francisco State University. She was Director for the Society for Industrial Microbiology (SIM). She received the ASM and California Hayward outstanding educator awards and SACNAS Mentor award. In addition to teaching, Chris contributes regularly to professional literature, develops innovative educational methodologies, and maintains a personal and professional commitment to conservation and the importance of science in society. Chris is also an avid photographer, and many of her photographs appear in this lab manual.

Key Benefit: Containing 57 thoroughly class-tested exercises, this manual provides basic microbiology techniques with applications for undergraduate readers in diverse areas, including the biological sciences, the allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The Ninth Edition features a new, four-color design and a dramatically new art program. Many of the illustrations have been re-rendered in a modern, realistic, three-dimensional style, and detailed, colorful photomicrographs that were once grouped together in a color insert are now integrated throughout the exercises. Experiments have been refined throughout, and a new exercise on parasitic helminths readers with valuable practice in microscopic examination and observation.

Key Topics: Use and Care of the Microscope, Examination of Living Microorganisms, Microbes in the Environment, Transfer of Bacteria: Aseptic Techniques, Preparation of Smears and Simple Staining, Negative Staining, Gram Staining, Acid-fast Staining,

Structural Stains (Endospore, Capsule, Flagella), Morphologic Unknown, Isolation of Bacteria by Dilution Technique, Special Media for Isolating Bacteria, Carbohydrate Catabolism, Fermentation, Protein Catabolism, Part 1, Protein Catabolism, Part 2, Respiration,

Unknown Identification and Bergey’s Manual, Oxygen and the Growth of Bacteria, Determination of a Bacterial Growth Curve: The Role of Temperature, Biofilms, Physical Methods of Control: Heat, Physical Methods of Control: Ultraviolet Radiation, Chemical Methods of Control: Disinfectants and Antiseptics, Chemical Methods of Control: Antimicrobial Drugs, Effectiveness of Hand Scrubbing, Regulation of Gene Expression, Isolation of Bacterial Mutants, Transformation of Bacteria, DNA Fingerprinting,

Genetic Engineering, Ames Test for Detecting Possible Chemical Carcinogens, Fungi: Yeasts and Molds, Phototrophs: Algae and Cyanobacteria 35. Protozoa, Parasitic Helminths, Isolation and Titration of Bacteriophages, Plant Viruses, Epidemiology, Koch’s Postulate, Nonspecific Resistance, Blood Group Determination: Slide Agglutination, Agglutination Reactions: Microtiter Agglutination, ELISA Technique, Bacteria of the Skin, Bacteria of the Respiratory Tract,Bacteria of the Mouth, Bacteria of the Gastrointestinal Tract, Bacteria of the Genitourinary Tract, Rapid Identification Methods, Identification of an Unknown from a Clinical Sample, Microbes in Water: Multiple-Tube Technique, Microbes in Water: Membrane Filter Technique,

Microbes in Food: Contamination, Microbes Used in the Production of Foods, Microbes in Soil: The Nitrogen and Sulfur Cycles,

Microbes in Soil: Bioremediaton

Market: Intended for those interested in learning the basics of microbiology

Booknews

A laboratory companion to , Sixth Edition by Tortora, Funke, and Case. The manual contains 56 class- tested exercises, including all those recommended by the Committee on Undergraduate and Graduate Education of the American Society for Microbiology. Sections cover microscopy, staining methods, cultivation of bacteria, microbial metabolism, microbial growth, control of microbial growth, microbial genetics, the microbial world, viruses, interaction of microbe and host, immunology, microorganisms and disease, and microbiology and the environment. A color photo quiz requires students to identify an unknown. Spiral wire binding. Annotation c. by Book News, Inc., Portland, Or.

Introduction

I. MICROSCOPY

1. Use and Care of the Microscope

2. Examination of Living Microorganisms

II. Handling Bacteria

3. Microbes in the Environment

4. Transfer of Bacteria: Aseptic Techniques

III. STAINING METHODS

5. Preparation of Smears and Simple Staining

6. Negative Staining

7. Gram Staining

8. Acid-fast Staining

9. Structural Stains (Endospore, Capsule, Flagella)

10. Morphologic Unknown

IV. CULTIVATION OF BACTERIA.

11. Isolation of Bacteria by Dilution Technique

12. Special Media for Isolating Bacteria

V. MICROBIAL METABOLISM

13. Carbohydrate Catabolism

14. Fermentation

15. Protein Catabolism, Part 1

16. Protein Catabolism, Part 2

17. Respiration

18. Unknown Identification and Bergey’s Manual

VI. MICROBIAL GROWTH

19. Oxygen and the Growth of Bacteria

20. Determination of a Bacterial Growth Curve: The Role of Temperature

21. Biofilms

VII. CONTROL OF MICROBIAL GROWTH

22. Physical Methods of Control: Heat

23. Physical Methods of Control: Ultraviolet Radiation

24. Chemical Methods of Control: Disinfectants and Antiseptics

25. Chemical Methods of Control: Antimicrobial Drugs

26. Effectiveness of Hand Scrubbing

VIII. MICROBIAL GENETICS

27. Regulation of Gene Expression

28. Isolation of Bacterial Mutants

29. Transformation of Bacteria

30. DNA Fingerprinting

31. Genetic Engineering

32. Ames Test for Detecting Possible Chemical Carcinogens

IX. THE MICROBIAL WORLD

33. Fungi: Yeasts and Molds

34. Phototrophs: Algae and Cyanobacteria 35. Protozoa

36. Parasitic Helminths

X. VIRUSES

37. Isolation and Titration of Bacteriophages

38. Plant Viruses

XI. INTERACTION OF MICROBE AND HOST

39. Epidemiology

40. Koch’s Postulate

XII. IMMUNOLOGY

41. Nonspecific Resistance

42. Blood Group Determination: Slide Agglutination

43. Agglutination Reactions: Microtiter Agglutination

44. ELISA Technique

XIII. MICROORGANISMS AND DISEASE

45. Bacteria of the Skin

46. Bacteria of the Respiratory Tract

47. Bacteria of the Mouth

48. Bacteria of the Gastrointestinal Tract

49. Bacteria of the Genitourinary Tract

50. Rapid Identification Methods

51. Identification of an Unknown from a Clinical Sample

XIV. MICROBIOLOGY AND THE ENVIRONMENT

52. Microbes in Water: Multiple-Tube Technique

53. Microbes in Water: Membrane Filter Technique

54. Microbes in Food: Contamination

55. Microbes Used in the Production of Foods

56. Microbes in Soil: The Nitrogen and Sulfur Cycles

57. Microbes in Soil: Bioremediaton

APPENDICES

A. Pipetting

B. Dilution Techniques and Calculations

C. Use of the Spectrophotometer

D. Graphing

E. Use of the Dissecting Membrane

F. Use of the Membrane Filter

G. Electrophoresis

H. Keys to Bacteria

Index